cfDNA Analysis: From Plasma to Variants


As the cost of sequencing continues to drop, the throughput and complexity of NGS assays have risen precipitously. At the same time, the types of samples being used for these assays have expanded as researchers find value in studying low-input, degraded, biological samples, such as cell-free DNA (cfDNA). In this webinar, Integrated DNA Technologies presents a complete workflow for effective analysis of <1% variant allele frequency (VAF) in cfDNA.

In this webinar, we will:

  • Describe how to increase cfDNA extraction efficiency using magnetic bead–based technology.
  • Demonstrate how to maximize conversion, coverage and complexity from low inputs of cfDNA during library preparation.
  • Utilize fixed UMIs for bioinformatic error correction to reduce sequencing and PCR errors.
  • Discuss how to increase throughput, reduce hands-on time, and minimize errors during complex NGS assays.

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